CAS number：84603-62-3
molecular formula：C8H4N2O4
molecular weight：
EINECS number：283-263-6

English synonyms

Lonicerae Japonicae Flos。;Honeysuckle, Lonicera caprifolium, ext.;LONICERA CAPRIFOLIUM (HONEYSUCKLE) FLOWER EXTRACT;Japanese Honeysuckle P.E.;Einecs 283-263-6;Extract of honeysuckle;Lonicera;Lonicera extract

Related categories

Plant extracts;Standard extracts;Ratio extracts;Plant extracts;Standard extracts;Extracts;

Introduction

Honeysuckle is a common traditional Chinese medicine in my country, which is the dried flower bud of Lonicera japonica Thunb. The honeysuckle extract obtained after extraction and drying of honeysuckle has been widely used in Chinese patent medicines such as Yinhuang Oral Liquid, Yinhuang Capsule, and Yinhuang Granule. Chlorogenic acid, the main active ingredient in honeysuckle extract, has pharmacological effects such as anti-virus, improving immunity, and lowering blood lipids. Honeysuckle extract has been gradually applied in the fields of pharmacy, fragrance, cosmetics, health food, health drink and so on, and the prospect is very broad.

Use

●  Anti-inflammatory, antipyretic, anti-allergic effects
Honeysuckle extract can inhibit rat carrageenan-induced swollen feet and reduce the degree of egg white-induced swollen feet. It also has obvious anti-exudation and anti-proliferative effects on croton oil granulation sacs in rats. Honeysuckle has obvious antipyretic effect on experimental animal fever model. Yinqiao Powder has a significant inhibitory effect on a variety of experimental inflammations, especially allergic inflammations, and can significantly inhibit the fever in rats caused by 2,4-dinitrophenol.

●  Antifertility
The decoction after the ethanol extraction of honeysuckle has obvious anti-fertility effect by parenteral administration. It is effective for early, middle and late pregnancy in mice when injected intraperitoneally, and is also effective for early pregnancy in rabbits by intravenous drip or intrauterine administration. Honeysuckle can significantly reduce plasma progesterone in early-pregnant rats, and the anti-early pregnancy effect of honeysuckle can be completely canceled by exogenous progesterone and chorionic gonadotropin (HCG). formation, which can also be counteracted by progesterone.

●  Anti-oxidize effect
The water extract of honeysuckle has a direct elimination effect on hydrogen peroxide (H2O2) in vitro with a linear dose-effect relationship, but whether it also has an effect in vivo remains to be further studied. In addition, honeysuckle can improve the release of H2O2 from neutrophils in scalded mice to a certain extent, and can reduce the ability of neutrophils to synthesize and release lysosomal enzymes in scalded mice, indicating that it has an antioxidant effect. The experimental results show that the crude extract of chlorogenic acid and crude flavonoids can significantly inhibit the peroxidation of oil. Honeysuckle and chlorogenic acid are two phenolic acid compounds with high content in honeysuckle leaves. Phenolic acid is a typical compound with free radical scavenging effect, while honeysuckle is slightly stronger than chlorogenic acid. The DPPH method was used to study the antioxidant activity of Shan Yinhua, and the antioxidant activity was expressed by the inhibition rate. The inhibition rate was 95.4% when the concentration of Shan Yinhua was 5 mg/ml, and the inhibition rate was 94.3% when the extract was diluted 5 times, i.e. the concentration was 1 mg/ml, indicating that Shan Yinhua had strong antioxidant capacity.

●  Liver protection and gallbladder
The chlorogenic acid contained in honeysuckle extract has a choleretic effect and can increase bile secretion in rats. Subcutaneous injection of 200mg/kg of total saponins from Lonicera japonica can significantly resist the increase of serum GPT and the content of hepatic triglyceride in the liver of mice caused by CCl4, paracetamol and galactosamine, and significantly reduce the severity of liver pathological damage. The sum of the number of punctate necrosis and the occurrence rate of necrosis were significantly reduced.

●  Lower blood lipids, enhance immune function
Rat gavage with honeysuckle extract 2.5g/kg can reduce the absorption of cholesterol in the intestine and reduce the content of cholesterol in the plasma. In vitro tests have also found that honeysuckle can bind to cholesterol. The total saponins of Lonicera japonica can significantly reduce the content of triglyceride in the liver of normal mice, and also greatly reduce the content of triglyceride in the liver of mice poisoned by CCl4, paracetamol and galactosamine. Honeysuckle water extract can promote the phagocytosis of peripheral blood leukocytes. Intraperitoneal injection of honeysuckle injection in mice also significantly promoted the phagocytosis of inflammatory cells. 250mg/kg honeysuckle water extract can reduce the positive percentage of T cells ANAE, suggesting that it may have inhibitory effect on cellular immunity. In addition, chlorogenic acid also has the effect of increasing white blood cells as the main active ingredient of ramie.

●  hemostatic effect
The chlorogenic acid and caffeic acid contained in the honeysuckle extract have significant hemostatic effects, which can shorten the coagulation time and bleeding time. Chlorogenic acid and caffeic acid are also the effective hemostatic components of ramie root. Chlorogenic acid is not resistant to high temperature, and it will be destroyed when heated for a long time and its efficacy will be reduced. Silver flower charcoal is traditionally used to stop bleeding, which may be related to this.

●  central excitatory effect
Oral administration of chlorogenic acid can excite the central nervous system of animals such as rats and mice, and its intensity is 1/6 of that of caffeine.

●  Antibacterial and antiviral effects
Honeysuckle extract has a wide antibacterial range and has inhibitory effects on gram-positive bacteria such as Staphylococcus aureus, Streptococcus hemolyticus, Streptococcus pneumoniae, Bacillus pertussis, and Shigella Shigella, Typhoid Bacillus, Paratyphoid Bacillus, Cholera Vibrio, Pseudomonas aeruginosa, Escherichia coli, Mycobacterium tuberculosis, meningococcus and other gram-negative bacteria also have strong inhibitory effects, and are also effective against Leptospira. The decoction of this product has in vitro inhibitory effect on various dermatophytes such as Trichophyton violaceum, Fusarium xylophilus, Microsporum rust, Epidermophyton rubrum, Nocardia stellaris, etc. Recent studies have shown that honeysuckle has antibacterial effects on common pathogenic bacteria such as Streptococcus mutans, Actinobacillus actinomyces, Bacteroides gingivalis, and Bacillus melanogenum that cause oral diseases. Honeysuckle or its compound has inhibitory effect on respiratory viruses such as influenza Asian type A and orphan virus, and can inhibit and delay their cytopathic effects. In addition, it also has a certain inhibitory effect on the herpes virus. It is generally believed that its main active ingredients are chlorogenic acid compounds.

●  Other role
In vitro tests of honeysuckle in water and alcohol have obvious cytotoxic effects on mouse sarcoma S180 and Ehrlich ascites carcinoma. Oral administration of honeysuckle extract has mild preventive effect on pylorus ligated gastric ulcer in rats. Oral administration of large doses of chlorogenic acid can enhance gastrointestinal motility and promote gastric juice and bile secretion. Chlorogenic acid and its decomposition products have excitatory effects on the isolated rat uterus. Chlorogenic acid contained in honeysuckle can cause central nervous system excitation in rats and mice. In addition, chlorogenic acid slightly enhanced the vasopressor effects of epinephrine and norepinephrine in cats and rats, but had no effect on the cat's nictitating membrane response.

Production method

●  Take 1kg of honeysuckle by the lime sulfur method, add water to decoct twice, 2 hours each time, combine the decoction liquid, filter, add lime milk to the filtrate to adjust the pH to 10-12, let it stand, filter out the precipitate, add an appropriate amount of water, and adjust the pH with sulfuric acid The filtrate is concentrated to a thick paste and spray-dried to obtain the honeysuckle extract.

●  Take 1kg of honeysuckle by the modified lime sulfur method, add (15, 10) times the amount of water, extract twice at 80-90°C for 2 hours each, filter, combine the filtrates, and concentrate the filtrate to 2/5 of the original volume, add 20 % lime milk to adjust the pH value to 10-12, let stand for 12h, filter out the precipitate, add 2 times the amount of 95% ethanol to suspend, add 50% sulfuric acid to adjust the pH value to 3-4, mix well, filter, and use 40% filtrate The pH value was adjusted to 6-7 with NaOH, filtered, concentrated to a thick paste, and spray-dried to obtain the honeysuckle extract.

●  Ultrafiltration treatment Take 1kg of honeysuckle, add (15, 10) times the amount of water, decoct twice, 2h each time, filter, combine the filtrates, concentrate the filtrate to 2/5 of the original volume, let it stand, and filter , ultrafiltration, collecting the ultrafiltrate, concentrating to the crude drug amount, standing, filtering, concentrating under reduced pressure, and vacuum drying to obtain the honeysuckle extract.

●  Reflux extraction with ethanol: Weigh 1 kg of honeysuckle, put it in a round-bottomed flask, add (10, 8) times the amount of 70% ethanol, heat under reflux for 2 times, 2h each time, filter, combine the filtrates, concentrate under reduced pressure, and vacuum dry to obtain honeysuckle extraction thing.

●  Macroporous adsorption resin treatment Take 1 kg of honeysuckle, add (15, 10) times the amount of water, extract twice at 90 °C for 2 h each time, filter, combine the filtrates, add the filtrate to the pretreated macroporous adsorption resin, add water Elution, the eluate was collected, concentrated under reduced pressure, and dried in vacuo to obtain the honeysuckle extract.

Team Presentation